Validated Edit-R Lentiviral sgRNA Positive Controls and kits confirm generation of insertions and deletions (indels), and permit quantification of gene editing efficiency using DNA mismatch detection assays.
Edit-R Lentiviral sgRNA Positive Control Kits include:
Lentiviral particles or glycerol stock
DNA mismatch detection primers (forward and reverse)
The Edit-R Lentiviral CRISPR-Cas9 Gene Engineering platform includes two critical components based on the natural S. pyogenes system: a vector for Cas9 expression and a gene-specific vector for sgRNA expression designed to the target site of interest. To facilitate rapid generation of cells lines that constitutively express Cas9 nuclease and the sgRNA, the Cas9 and sgRNA vectors are available pre-packaged into lentiviral particles that have been purified and concentrated. Cas9-expressing cells lines can be easily generated with the Edit-R Lentiviral Cas9 Nuclease Expression particles and subsequent gene knockouts can be obtained by an additional transduction with sgRNA lentiviral particles.
Caracteristicas
Control lentiviral sgRNAs to verify DNA double-strand breaks and gene editing efficiencies.
