Controles Edición Génica -Edit-R Predesigned Lentiviral sgRNA - Mouse

Disponible
Predesigned with an algorithm to improve functional knockout and best-in-class specificity checking. Dharmacon single guide RNAs enable high-confidence gene editing experiments without the need for laborious cloning or lentiviral packaging.
Aplicaciones | Edición génica |
---|---|
Categoría de Producto | Controles CRISPR |
Subcategoría de Producto | Lentiviral |
Disponibilidad | Bajo pedido |
Especie/Huesped | Ratón |
Ref Dutscher | 77GSGM11839 |
Especificaciones | The Edit-R algorithm's alignment tool identifies mismatches AND gaps to optimize selection of highly specific target sequences; and The Edit-R algorithm scores were developed on functional gene knockout rather than just quantifying indels in the genomic target DNA. Pre-designed Edit-R Lentiviral sgRNAs are supplied as glycerol stocks and as concentrated high-titer particles for straightforward delivery into Cas9-expressing cells for efficient knockout without need for cloning or packaging. Purified, concentrated lentiviral particles can be directly transduced into difficult-to-transfect cells without the toxic cellular debris and contaminants found in supernatant preparations.Glycerol stocks can be grown and the expression plasmid purifed for immediate transfection or packaging into particles. Save money by eliminating time-consuming cloning, packaging and titering steps High-quality, concentrated, purified lentiviral particles for direct transduction with minimal cytotoxicity Pre-designed sgRNA generated using the Edit-R CRISPR RNA algorithm for unparalleled specificity and functionality |
Caracteristicas | Genome-wide single guide RNAs for efficient gene knockout & unparalleled specificity; available as glycerol stocks and high-titer purified particles |
Marca | HORIZON |
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