Controles Expresión Génica -Edit-R CRISPRa Pou5f1 Lentiviral sgRNA Positive Control - Human

Edit-R CRISPRa Lentiviral sgRNA Positive controls are designed to specifically activate the human and mouse Titin (TTN) or POU class 5 homeobox 1 (POU5F1) genes. It is recommended that positive controls always be used to optimize experimental conditions and included in every gene activation experiment to confirm successful delivery and dCas9-VPR activity. The level of activation observed will depend on your cell type and basal level of expression. If either TTN or POU5F1 is already expressed in your cells, there will be more modest activation than with a gene that is not expressed. Normalization of the activation level to a non-targeting control will provide a baseline for determining optimal dCas9-VPR expression, transfection efficiency, and timepoint for your assay.