Edit_R EGFP Cas9 Nuclease mRNA

Edit-R Cas9 Nuclease mRNA expresses a human codon-optimized version of the S. pyogenes Cas9 gene with two nuclear localization signals (NLS). The Cas9 endonuclease complexes with a guide RNA for genomic DNA target recognition and cleavage. Edit-R Cas9 Nuclease mRNA, in conjunction with Edit-R synthetic sgRNA or crRNA:tracrRNA, enables researchers to perform genome engineering experiments in a completely DNA-free manner. The Cas9 Nuclease mRNA is available in a form that co-expresses either mKate2 or EGFP for transfection optimization or enrichment using fluorescence-activated cell sorting (FACS). The benefits to this approach are: