Efficient two-vector system that utilizes a lentiviral vector for Cas9 expression and a gene-specific vector for sgRNA expression
Rationally designed Edit-R lentiviral sgRNAs efficient gene knockout with unparalleled specificity using a functionally validated proprietary algorithm
Deep and broad coverage of 5 -10 sgRNAs per gene across the human, mouse and rat genomes for increased hit confidence and comprehensive genome screening
Can be combined with the promoter flexibility of Edit-R Lentiviral Cas9 Nuclease Reagents for robust editing in biologically relevant cell types
Each Edit-R Lentiviral sgRNA Pooled Screening Library includes
≥ 5 x 108 TU/mL ( ± 20%) lentiviral particles in pre-aliquoted tubes 8 x 25 µL (200 µL total) for libraries ≤ 5000 constructs
16 x 25 µL (400 µL total) for libraries > 5000 constructs
Five to ten sgRNAs per gene targeting coding genes in the NCBI Reference Sequence Database
100 non-targeting sgRNA negative controls bioinformatically confirmed to not align with (target) any gene in the human, mouse and rat genomes.
Up to 340 gene-specific sgRNA positive controls targeting up to 34 protein-coding genes (10 sgRNA per gene); including common reference genes (ACTB, GAPDH, LAMB1, & PPIB)
A data file containing complete library information, including: gene annotations, sgRNA target sequences, complete list of controls, and counts per millions of mapped reads
Edit-R Lentiviral sgRNA libraries are available for defined sub-libraries of gene families up to the entire genome for human and mouse; rat libraries and custom Edit-R Lentiviral sgRNA collections are available upon request.
Request a custom collection of human, mouse, or rat gene-targeting sgRNAs
Available in pool sizes between 50 to 10,000 constructs
Pools are provided as purified, concentrated lentiviral particles ≥ 5 x 108 TU/mL
Caracteristicas
Whole genome, druggable genome, gene family and pathway lentiviral sgRNA pooled libraries for knockout screening.
