Kit de optimización de transfección de células primarias para Nucleofector 96 pocillos Shuttle, 1 kit de 96 reacciones

The Primary Cell Optimization 96-well Shuttle^TM Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well Shuttle^TM Add-On.

With the unique capability of the 96-well Shuttle^TM Add-On to address individual wells of a 96-well Nucleocuvette^TM Plate with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using one 96-well Nucleocuvette^TM Plate and our pmaxGFP^TMVector as positive control, our five Primary Cell Nucleofector^TM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 32 wells of the second 96-well Nucleocuvette^TM Plate can be used for optional fine tuning or performing the first experiment with the selected conditions.

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector^TM Solutions and Nucleofector^TMPrograms. Basic Protocols are available for following cell groups:

• Endothelial cells (mammalian)
• Epithelial cells (mammalian)
• Fibroblasts (mammalian)
• Glial cells (mammalian)
• Neurons (mammalian)
• Smooth muscle cells (mammalian)
• Stem cells (human)

Benefits

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
• Optimal Nucleofection conditions determined on the 96-well Shuttle^TM Add-On are transferable to the 4D-Nucleofector^TM System or the 384-well HT Nucleofector^TMSystem

Applications

• Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 96-well Shuttle^TM Add-On