Kit de transfección básico de células primarias epiteliales de mamifero para Nucleofector 2b, 1 kit 25 reacciones

Content

2.25 mL Nucleofector^TM Solution for Primary Mammalian Epithelial Cells
0.5 mL Supplement 1
30 µg pmaxGFP^TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes

Nucleofector Kits for Mammalian Epithelial Cells:

• For mammalian epithelial cells lacking a cell-type specific Amaxa Optimized Protocol, we offer a selection of kits that can be used to easily define optimal Nucleofection Conditions.
• The P1 and P3 Primary Cell Kits together with the cellgroup specific Basic Protocols are suited for optimizations of mammalian epithelial cells on the 4D-Nucleofector and the 96-well Shuttle System.
• A cell group-specific Basic Kit is suited for optimization of mammalian epithelial cells using the Nucleofector II/2b Device.

Benefits:

• Optimizations can be performed within 1 experiment,
• Detailed protocols guiding through the optimization procedure,
• Optional result fine tuning with help of our Scientific Support Team,
• Transfection efficiencies up to 83%, Viabilities up to 98%.

Applications: 

• Kits suited for epithelial cells from different mammalian species and various organs, 
• Already tested for renal proximal tubular epithelial cells (RPTEC),
• Clonetics Epithelial Cells: human prostate epithelial cells (hPrEc) and human small airway epithelial cells (SAEC).
• For the 4D-Nucleofector Kits "L" and "S" indicate the Nucleocuvette Vessel format. L = 100 µl single Nucleocuvette, S = 16-well Nucleocuvette Strip.