MGC cDNA sequences are deposited in GenBank, and the clones can be purchased
Genome-scale coverage for human, mouse, rat, and cow
Shipped as a live bacterial culture containing plasmid of interest
Inserts match the corresponding GenBank BC accession number*
New - MGC Sequence Verified Human cDNA Clones
Now offering Sequence Verified Human MGC cDNAs from the Mammalian Gene Collection. This is the same collection developed by the National Institute of Health (NIH) but clone identities have now been sequence verified . This provides our customers with the most extensive and rigorously verified collection of mammalian cDNA clones available.
Human MGC cDNA Highlights
Based on valued customer feedback, we initiated a re-sequencing effort with the goal of confirming the BC Accession identity of the clones in the Human MGC collection.
As a result of this sequencing effort, we now provide the most accurate Human MGC cDNA clone collection on the market
The newly verified cDNA clones are now available with the catalog prefix MHS6278
If your human MGC clone does not have a catalog number starting with MHS6278, it has not yet been included in the re-sequencing effort and can still be located under the MGC Fully Sequenced offering (contact us for assistance - ts.dharmacon@ge.com)
Custom cDNA Libraries
Custom libraries are available. Please email requests to Technical Support, or call 1-800-235-9880. International customers, please call 303-604-9499 or your local Sales Representative.
About the MGC
The goal of the Mammalian Gene Collection (MGC), a trans-NIH initiative, is to provide researchers with unrestricted access to sequence-validated full-length protein-coding (FL-CDS) cDNA clones for human, mouse, and rat genes. In 2005, the project added the cow cDNAs generated by Genome Canada.
MGC clones initially were obtained by random EST-screening of cDNA libraries. Starting in 2004, MGC used transcript-specific RT-PCR cloning to isolate cDNAs for genes not represented in MGC. Finally, between 2006 and 2009, MGC employed DNA synthesis methods to provide FL-CDS clones for human and mouse genes still absent from the collection.
All MGC sequences are deposited in GenBank, and the clones can be purchased. You can use "A Guide to Finding Mammalian Gene Collection (MGC) Clones and Evaluating Their Sequence" to assist in determining whether MGC cDNA clones for human, mouse, or rat genes and transcripts of interest are available for purchase or sequence investigation.
With the conclusion of the MGC project in March 2009, the GenBank records of MGC sequences were frozen, without further updates. Since the definition of what constitutes a full-length coding region for some of the genes and transcripts for which we have MGC clones will likely change in the future, users planning to order MGC clones will need to monitor for these changes. Users can make use of genome browsers and gene-specific databases, such as the UCSC Genome browser, NCBI’s Map Viewer, and Entrez Gene, to view the relevant regions of the genome (browsers) or gene-related information (Entrez Gene).
Caracteristicas
Human, mouse, rat, and bovine cDNAs from the Mammalian Gene Collection (MGC)
