Choose from two universal miRIDIAN Mimic Negative Controls for experiments with miRIDIAN microARN Mimics. Negative control sequences based on C. elegans microARNs have minimal sequence identity in human, mouse, and rat.
Same structure and design as experimental miRIDIAN microARN Mimics
Target the 3' UTR (untranslated region) of the standard housekeeping genes, PPIB, or GAPDH (see Figure 1, Supporting Data)
Allows a clean, straightforward, cleavage-based readout of mimic function
Caracteristicas
Validated miRIDIAN microARN Mimics designed to target PPIB in human, mouse, and rat Delivery and function can be evaluated to determine transfection conditions in each cell line/type
Monitor the function of a mimic molecule at the marn level using standard transcript quantification methods, such as RT-qPCR