ORF strains provide a shortcut to expressing yeast proteins with no PCR or cloning required
The optional E. coli format enables Gateway-adapted cloning to alternative yeast strains and plasmids
All yeast-format constructs have been verified by western blotting to express protein of the correct length
The versatile 19-kDa tandem fusion tag enables a range of detection and affinity purification strategies. After cleavage with 3C protease, purified proteins are left with a 4.8kDa C-terminal tag
