Guaranteed gene silencing and patented modifications to reduce off-targets; available as pre-designed ON-TARGETplus SMARTpool and siRNA reagents for human, mouse or rat.
Highlights
Off-targets reduced by up to 90% compared to unmodified siRNA
Guaranteed silencing by SMARTpool and 3 of 4 individual siRNAs (see Specifications tab)
Sequence information provided with siRNA purchase
A two-stranded mechanism requires a two-stranded solution
Dharmacon scientists and collaborators demonstrated in 2006 (see Reference 1) that siRNA off-targets are mediated by antisense seed-region interactions, initiating the development of a dual-strand modification pattern:
Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
Seed-region analysis on siRNA designs reduces miRNA-induced off-targets
A landmark publication (see Reference 2) from the Dharmacon research group was the first to experimentally demonstrate the key role of the seed region in mediating off-targets. A subsequent 2008 paper (see Reference 3) showed the importance of seed frequency in the 3'UTR as in indicator of its likelihood to cause off-targets. These principles were subsequently applied to our siRNA designs to improve specificity:
Design filters eliminate common seed regions likely to cause miRNA-like off-targets
Seed frequency analysis for siRNA designs minimize off-target effects
Available Product Formats
SMARTpool: A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus guaranteed to silence by 75% or better (see Specifications).
Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene. Three of four siGENOME and ON-TARGETplus siRNAs are guaranteed to silence by 75% or better (see Specifications).
Set of 4 Upgrade: Discounted price on the Set of 4 based on a concurrent or prior purchase of the SMARTpool reagent for the same gene.
Individual siRNAs: Select 1, 2, or 3 individual siRNAs per gene. Minimum purchase of 4 at the 2 nmol size.
Caracteristicas
siRNA designed and modified for greater specificity
