Osteoblastos NHOst criopreservados

Most CloneticsPrimary Cells are also available as proliferating cells in either flasks or plates, or as pellets in RNALater. Cryopreserved CloneticsNormal Human Osteobalsts (NHOst) are guaranteed through 10 population doublings, stain positive for alkaline phosphatase and mineralization. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. CloneticsOGMOsteoblast Growth Medium is specially formulated for the growth and expansion of human osteoblasts. Contains 10% serum. OGMDifferentiation Supplements are recommended for differentiation. CloneticsMedia Products are specially designed to support the growth of human and animal primary derived cells. Media systems have been specifically developed and optimized for various cell systems. Media are supplied in several formats: Basal Medium - contains no growth factors cytokines, or supplements SingleQuotsKit- growth factors and supplements BulletKit- basal medium and SingleQuotsKit packaged together The OsteoImageMineralization Assay is a rapid, fluorescent in vitro assay for assessing bone cell mineralization. The OsteoImageAssay can quantitate in vitro mineralization by osteogenic stem cells, primary osteoblasts, and osteoblast-like cell lines. Osteoblasts are responsible for building bone matrix by producing mineral deposits. Their counterpart, Osteoclasts, are responsible for breaking down and resorbing bone. Osteoblasts mineralization can be measured in vitro using various assays including von Kossa, and Alizarin Red though Lonza recommends using the OsteoImageMineralization Assay for detecting mineral as it specifically binds to hydroxyapatite.