Rabbit anti Human CAD antibody recognizes the DNA fragmentation factor subunit beta, also known as DNA fragmentation factor 40 kDa subunit, caspase-activated DNase, DFF40, CPAN or caspase-activated nuclease.
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for DFFB but the biological validity of some of these variants has not been determined (provided by RefSeq, Sep 2013).
Rabbit anti Human CAD antibody detects a band of 46 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
