RABBIT ANTI FIP1L1

Rabbit anti Human FIP1L1 antibody recognizes FIP1L1, also known as Pre-mRNA 3'-end-processing factor FIP1, FIP1-like 1 protein and factor interacting with PAP.

The FIP1L1 gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. FIP1L1, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. Its N-terminus contains a PAP-binding site and its C-terminus an RNA-binding domain. An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that joins the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. FIP1L1 fusion and chromosomal deletion is the cause of some forms of idiopathic hypereosinophilic syndrome (HES). This syndrome, recently reclassified as chronic eosinophilic leukemia (CEL), is responsive to treatment with tyrosine kinase inhibitors. Alternative splicing results in multiple transcript variants encoding distinct isoforms (provided by RefSeq, Oct 2008).

Rabbit anti Human FIP1L1 antibody detects a band of 77 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.