RABBIT ANTI IRP1

Rabbit anti Human IRP1 antibody recognizes IRP1, also known as IRE-BP 1, citrate hydro-lyase, ACO1 and cytoplasmic aconitate hydratase.

The protein encoded by ACO1 is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. Alternative splicing results in multiple transcript variants (provided by RefSeq, May 2013).

Rabbit anti Human IRP1 antibody detects a band of 98 kDa. The antibody has been extensively validated for western blotting using whole cell lysate.