RABBIT ANTI WILMS TUMOR 1

Rabbit anti Human Wilm's tumor 1 antibody recognizes Wilms tumor 1, also known as WT1, amino-terminal domain of EWS or WT33.

WT1 gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. WT1 exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. WT1 mRNA undergoes RNA editing in human and rat, and this process is tissue-restricted and developmentally regulated (Sharma et al. 1994) (provided by RefSeq, Oct 2010).

Rabbit anti Human Wilm's tumor 1 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.