SYBR Green I stain is a highly sensitive fluorescent stain for detecting dsDNA and oligonucleotides in agarose and polyacrylamide gels. Because SYBR Green's fluorescent signal is greatly enhanced when the dye is bound to nucleic acids, gels stained with SYBR Green I exhibit an excellent signal-to-noise ratio with no background fluorescence. For maximum performance, gels should be poststained. SYBR Green I is ideal for gel-based detection of small amounts of DNA in low copy/target number PCR products, apoptosis studies, and heteroduplex analysis.
Detection Limits
dsDNA at 300nm transillumination: 60 pg
Oligonucleotides at 300nm transillumination 1-2 ng
