Highlights
Selection using blasticidin resistance marker (BlastR) or fluorescent marker (mKate2 or TurboGFP™)
Provided as concentrated, purified lentiviral particles for immediate transduction, with a minimum ≥ 1 × 107 TU/mL functional titer, by qPCR titering.
Also available as certified endotoxin-free plasmid DNA for direct transfection into a packaging cell line and production of your own lentiviral particles
Customize your construct with one of six SMARTchoice constitutive promoters to ensure optimal Cas9 expression in your cell line of interest
Utilize the tight regulation of the inducible Cas9 vector when you require temporal control over the expression of Cas9 or to generate a stable cell line with minimal background expression.
Not all RNA pol II promoters are equally active in different cellular environments
The activity of any given promoter controlling the transcription of Cas9 nuclease can differ greatly from one biological context to another, resulting in variable Cas9 expression levels and thus varying levels of DNA cleavage. Choosing an optimal promoter for your cell line or type will therefore affect the degree of gene editing in your experiment.
SMARTchoice promoter options for expressing Cas9 nuclease
Promoter
Description
hCMV human cytomegalovirus immediate early promoter
mCMV mouse cytomegalovirus immediate early promoter
hEF1α human elongation factor 1 alpha promoter
mEF1α mouse elongation factor 1 alpha promoter
PGK mouse phosphoglycerate kinase promoter
CAG chicken beta actin hybrid promoter
TRE3G doxycycline-inducible promoter
Caracteristicas
Lentiviral CRISPR-Cas9 components for robust gene editing in biologically relevant cell types
