Reactivos Expresión Génica -Edit-R CRISPRa Lentiviral mCMV-Blast-dCas9-VPR Particles

Disponible
Edit-R CRISPRa Lentiviral mCMV-Blast-dCas9-VPR Particles
Aplicaciones | Expresión génica |
---|---|
Categoría de Producto | CRISPRa |
Subcategoría de Producto | Lentiviral |
Temperatura Envío | Congelado -20º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Ref Dutscher | 77VCAS11920 |
Especificaciones | Highlights Selection using blasticidin resistance marker (BlastR) or fluorescent marker (mKate2 or TurboGFP™) Provided as concentrated, purified lentiviral particles for immediate transduction, with a minimum ≥ 1 × 107 TU/mL functional titer, by qPCR titering. Also available as certified endotoxin-free plasmid DNA for direct transfection into a packaging cell line and production of your own lentiviral particles Customize your construct with one of six SMARTchoice constitutive promoters to ensure optimal Cas9 expression in your cell line of interest Utilize the tight regulation of the inducible Cas9 vector when you require temporal control over the expression of Cas9 or to generate a stable cell line with minimal background expression. Not all RNA pol II promoters are equally active in different cellular environments The activity of any given promoter controlling the transcription of Cas9 nuclease can differ greatly from one biological context to another, resulting in variable Cas9 expression levels and thus varying levels of DNA cleavage. Choosing an optimal promoter for your cell line or type will therefore affect the degree of gene editing in your experiment. SMARTchoice promoter options for expressing Cas9 nuclease Promoter Description hCMV human cytomegalovirus immediate early promoter mCMV mouse cytomegalovirus immediate early promoter hEF1α human elongation factor 1 alpha promoter mEF1α mouse elongation factor 1 alpha promoter PGK mouse phosphoglycerate kinase promoter CAG chicken beta actin hybrid promoter TRE3G doxycycline-inducible promoter |
Caracteristicas | Lentiviral CRISPR-Cas9 components for robust gene editing in biologically relevant cell types |
Marca | HORIZON |
An error has happened during application run. See var/log/wp/error.log for details
Recomendaciones
-
-
shMIMIC Lentiviral microRNA Pooled Library -miRBas8.512,42 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Non-targeting hEF1a-None Control Parti830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
CCSB-Broad Lentiviral Expression Nuclear Receptor1.405,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Reactivos Edición Génica -Edit-R HDR Plasmid Donor Kit - EGFP787,77 € sin ivaPara ver tus precios y comprar inicia sesión
-
-
miRIDIAN microRNA Mimic Negative Control #2298,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Non-targeting mCMV-TurboGFP Glycerol463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Mouse Inducible Phosphatase Lentiviral6.852,12 € sin ivaPara ver tus precios y comprar inicia sesión
-
siGENOME siRNA RTF - Cell Cycle Regulation -black plates - Mouse2.665,36 € sin ivaPara ver tus precios y comprar inicia sesión
-
-
Edit-R Mouse Dnmt3b Synthetic sgRNA Control Kit451,00 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Non-targeting hEF1a-None Glycerol 5463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Set of 3 SMARTvector Mouse Inducible LNC shRNA 1002.276,30 € sin ivaPara ver tus precios y comprar inicia sesión
-
siGENOME siRNA Library - Serine Proteases - Set of 4 - Mouse5.718,30 € sin ivaPara ver tus precios y comprar inicia sesión
-
-
Yeast W303 MATalpha Strain (glycerol stock)116,00 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Inducible Non-targeting hEF1a-TurboGFP830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Human Ubiquitin Conjugation Lentiviral5.710,95 € sin ivaPara ver tus precios y comprar inicia sesión