Genome-wide human and mouse lentiviral sgRNA reagents are specifically designed for CRISPRa. When used in conjunction with dCas9-VPR expression, you can leverage the power of the CRISPR-Cas9 system for activation of your favorite gene.
Caracteristicas
Individual constructs and sets of 4 are available as glycerol stocks and high-titer purified lentivirus particles
• Set-of-4 format ensures broad coverage of a target genes transcription start site, providing robust activation and expression
• Designed with a published algorithm by Horlbeck, et. al. (2016) that has demonstrated strong levels of gene activation from optimized designs adjacent to one or more transcription start sites
• Purified, concentrated high-titer lentivirus particles can be directly transduced into cells, including difficult-to-transfect cells and lines
