SET REACTIVOS ELISA, AAC PARA 480 ENSAYOS, 1 KIT

The DAS-ELISA reagents (1) were made against a blend of different Aac isolates originating from USA, Brazil, and Thailand (R. Walcott, pers. communication). The reagent is suitable for detecting Aac in cultures or in symptomatic plant leaves. All isolates of Aac tested so far can be detected. Isolates of Aac can be detected to a concentration of 104 cfu per ml (diluting bacteria from pure culture in extraction buffer). This detection limit is nearly reached when bacteria are diluted in sap of healthy watermelon leaves. There are cross-reactions with other subsp. of Acidovorax avenae, such as A. avenae subsp. cattleyae (Aacat) or A. avenae subsp. avenae (Aaa). Best results are obtained with an 1:20 (w/v) plant tissue extraction with extraction buffer «General» (Art. No. 110120).