SET REACTIVOS ELISA, AACAT PARA 960 ENSAYOS, 1 KIT

The DAS-ELISA reagents (1) were made against the Phalaenopsis isolate B108 of Aacat (W. Wohanka, pers. communication). The reagent is suitable for detecting Aacat in cultures or in symptomatic plant leaves (3). All isolates of Aacat tested so far can be detected. Isolates of Aacat can be detected to a concentration of 104 cfu per ml (diluting bacteria from pure culture in extraction buffer). This detection limit is nearly reached when bacteria are diluted in sap of healthy Phalaenopsis leaf. There are cross-reactions with other subsp. of Acidovorax avenae, such as Acidovorax avenae subsp. citrulli (Aac) or Acidovorax avenae subsp. avenae (Aaa). Best results are obtained with an 1:20 (w/v) plant tissue extraction with extraction buffer «General» (Art. No. 110120). The product was developed in cooperation with the State Research Institute Geisenheim, Germany. The development was partially supported by SWISSORCHID, Meyer Pflanzenkulturen, Wangen (ZH), Switzerland.