SET REACTIVOS ELISA, CAMV PARA 480 ENSAYOS, 1 KIT

The reagents were made against a Czech CaMV isolate, multiplied on Chinese cabbage (Brassica pekinensis), cv. Nozaki and react specifically with CaMV (4) in DAS-ELISA (1). Isolates from different host plants, e.g. from winter and spring rape, mustards, turnips, and cauliflower, are quite uniform regarding their virulence (3) and serological detection. All isolates of CaMV tested so far have been detected. Main affected crops are different species of Brassica. The concentration of the virus in plants is generally low and their particles are embedded in inclusion bodies. Test samples must be homogenized 1:100 (w/v) in extraction buffer «Caulimo» (Art. No. 110124). The special extraction buffer (details see below and next page) helps releasing virus particles and increases signal significantly in ELISA. The product was developed in cooperation with the Institute of Plant Molecular Biology, Ceske Budejovice, Czech Republic.