SFM - Medio especial mantenimiento células progenitoras neurales NP mM BulletKit, medio basal y suplementos, 1 kit

PoieticsNeural Progenitor Cells (NHNP) are cryopreserved neurospheres isolated from human brain cortex. Neurospheres are clusters of cells typically referred to as neural stem cells and progenitors (NSPCs) or Human midbrain-derived neural progenitor cells (hmNPCs). These neurospheres can be maintained undifferentiated in suspension as a 3D aggregate. To induce differentiation, the neurospheres can be transferred to laminin-coated vessels with brain-derived neurotrophic (BDNF) which will induce differentiation into neurons, astrocytes, and oligodendrocytes.

Recommended Medium: NPMMNeural Progenitor Maintenance BulletKitMedium for recovery from cryopreservation and maintenance as neurospheres; NPDMNeural Progenitor Differentiation BulletKitMedium + 25 ng/ml brain-derived neurotrophic factor (BDNF) for non-directed differentiation of neurospheres into neurons, astrocytes, and oligodendrocytes. Each bottle of the NPDMNeural Progenitor Differentiation BulletKitMedium contains enough differentiation medium to fully differentiate approximately 1.2 million neural progenitor cells [approximately 20 wells of a 24-well plates] into mature, functionally active neurons, astrocytes, and oligodendrocytes.

Lonza guarantees the performance of Clonetics/Poieticscells only if appropriate Clonetics/Poieticsmedia and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
Lonza offers 2 optimized media kits specially formulated to support the maintenance and differentiation of the NHNP cells:

NPMMNeural Progenitor Maintenance BulletKitMedium contains the necessary supplements and medium for recovery of Neural Progenitor (NHNP) neurospheres from cryopreservation and maintenance as neurospheres. This kit includes:

CC-3210 - NP Basal Medium
CC-4241 - NP Maintenance Medium SingleQuotsKit (contains hEGF and hFGF)
CC-4242 - NP Supplement SingleQuotsKit (contains NSF-1 and GA)

While it may be possible to promote some proliferation of Neural Progenitor (NHNP) neurospheres in the NPMMNeural Progenitor Maintenance BulletKitMedium, Lonza makes no claims with regards to the proliferative capacity of these cells.

NPDMNeural Progenitor Differentiation BulletKitMedium with the addition of 25 ng/ml brain-derived neurotrophic factor (BDNF) (not included) contains the necessary supplements and medium for optimal non-directed differentiation of Neural Progenitor (NHNP) neurospheres into neurons, astrocytes, and oligodendrocytes. The medium can be customized by supplementation with differentiating-promoting agents. This kit includes:

CC-3210 - NP Basal Medium
CC-4242 - NP Supplement SingleQuotsKit (contains NSF-1 and GA)

The basal medium and supplements can also be purchased individually.