microRNAs, endogenous non-coding ~22mer RNAs, are highly conserved in mammals and regulate gene expression post-transcriptionally through innate RNA interference mechanisms. Now, stable cell lines expressing microRNAs of interest can be created using novel vectors with your choice of promoter and reporter.
shMIMIC lentiviral microRNA designs are created for the robust over-expression of each mature human, mouse and rat microRNA in the miRBase database while minimizing the effects of the star (*), or passenger, strand. Inducible control of the shMIMIC microRNA and the reporter gene are conferred by the Tet 3G promoter (PTRE3G), while the expression of the Tet-On® 3G transactivator protein is under the control of a constitutive RNA pol II promoter.
The SMARTchoice inducible vector customization options include four inducible promoters (mCMV, PGK, hEF1α and mEF1α) and two reporter (TurboGFP and TurboRFP) options. shMIMIC Inducible Lentiviral microRNA designs are cloned and packaged into lentiviral particles on demand and provided as glycerol stocks and high-titer (≥ 1x107 TU/mL) viral particles
Caracteristicas
Innovative microRNA vector design coupled with your choice of optimal promoter and reporter for over-expression of mature microRNAs
• Your choice of four different constitutive promoters to optimize microRNA expression in your cells
• Design modifications promote preferential loading of the mature microRNA into RISC and robust function
• High-titer, concentrated lentiviral particles permits delivery into cells refractory to lipid-based delivery
• Expression of TurboGFP or Turbo RFP (Evrogen, Moscow) allows visualization of transduction efficiency; a no-reporter format is also available
• Glycerol stock format can be used to grow and isolate the lentiviral plasmid for direct transfection or packaging into lentiviral particles
