The RNAi Consortium, or TRC, is a public-private effort based at the Broad whose mission is to create a shRNA library to enable the scientific community to use RNAi to determine the function of human and mouse genes.
Rules-based shRNA design for efficient gene knockdown
Already cloned into lentiviral vectors
Amenable to in vitro and in vivo applications, such as the creation of stable knockdowns
Lentiviral vector enables transduction of primary and non-dividing cell lines
Broad coverage: 4 to 5 constructs per gene
Further information is available at http://www.broad.mit.edu/genome_bio/trc/rnai.html
Caracteristicas
Simple hairpin shRNAs in the pLKO.1 lentiviral vector designed by The RNAi Consortium (TRC)
