TRIPZ shRNA Packaging Starter Kit (glycerol kit)

Disponible
Study the function of essential genes, validate cellular phenotypes and generate inducible knockdown cell lines with TRIPZ Inducible Lentiviral shRNA.
Aplicaciones | Silenciamiento génico |
---|---|
Categoría de Producto | Empaquetamiento viral |
Temperatura Envío | Congelado -20º |
Temperatura Almacenaje | Congelado -80º |
Disponibilidad | Bajo pedido |
Ref Dutscher | 77RHS5087 |
Especificaciones | Tet-inducible shRNA expression permits reversible and regulatable gene knockdown microRNA-adapted shRNA design enhances specificity and potency of silencing TurboRFP fluorescent reporter allows visual tracking of shRNA expression Lentiviral vector enables transductions of primary and non-dividing cells Tet-On or Tet-Off configurations The pre-designed TRIPZ shRNA collection is configured for expression of an shRNA in the presence of doxycycline (Tet-On configuration). However, it is possible to alter the construct such that expression occurs in the absence of doxycycline (Tet-Off configuration). The pTRIPZ vector can be easily converted to a Tet-Off configuration by Cre-loxP site-specific recombination or a standard restriction digest. The rtTA3 is flanked by loxP sites allowing in vitro or in vivo excision of the rtTA3 by exposure to Cre recombinase. The rtTA3 is also flanked by a pair of BamHI restriction sites allowing for straightforward cleavage and ligation of the vector to remove the rtTA3. Without the rtTA3 present, a tetracycline transactivator (tTA) can be added extraneously to the system allowing it to function as Tet- Off®; where expression of shRNA and TurboRFP are alternatively induced in the absence of doxycycline. |
Caracteristicas | Inducible shRNA expression for regulatable gene silencing |
Marca | HORIZON |
An error has happened during application run. See var/log/wp/error.log for details
Recomendaciones
-
-
SMARTvector Rat GAPD hEF1a Control Particles830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Inducible Non-targeting hEF1a-TurboGFP830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Human Epigenetics Lentiviral shRNA Poo9.084,48 € sin ivaPara ver tus precios y comprar inicia sesión
-
ON-TARGETplus siRNA Library - Deubiquitinating Enzymes - SMARTpool - Human3.472,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
ON-TARGETplus GAPD Control Pool (Human)624,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Human GAPD Control shRNA hEF1a-RFP UHT2.868,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Non-targeting hEF1a-TurboRFP Control P830,71 € sin ivaPara ver tus precios y comprar inicia sesión
-
Controles Expresión Génica -Edit-R POU5F1 CRISPRa crRNA control pool - Human299,53 € sin ivaPara ver tus precios y comprar inicia sesión
-
-
Reactivos Expresión Génica -Edit-R CRISPRa Lentiviral mCMV-Blast-dCas9-VPR Plasmid DNA342,18 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Inducible Non-targeting hEF1a-TurboGFP463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Mouse Phosphatase Lentiviral shRNA Poo5.710,95 € sin ivaPara ver tus precios y comprar inicia sesión
-
-
Edit-R Synthetic sgRNA Non-targeting Control #3196,59 € sin ivaPara ver tus precios y comprar inicia sesión
-
SMARTvector Non-targeting hEF1a-TurboRFP Glycerol463,06 € sin ivaPara ver tus precios y comprar inicia sesión
-
Individual SMARTvector Mouse Lentiviral shRNA 2002.501,30 € sin ivaPara ver tus precios y comprar inicia sesión