The Yeast Tet-Promoters Hughes Collection (yTHC) with 800 essential yeast genes under control of a tetracycline-regulated promoter permits experimental regulation of essential genes.
Yeast Tet-Promoters Hughes Collection (yTHC) mutant strains are provided in the haploid MATa strain R1158
Each strain was qualified by its growth phenotype on YPD with 10 µg/mL doxycycline
In the absence of doxycycline, the Tet-promoter is fully activated
Addition of doxycycline in a titratable manner allows for down-regulation of the promoter until the gene of interest is no longer expressed at detectable levels
Background Strain = R1158 (derived from By4741)
Genotype of wildtype R1158 - URA3::CMV-tTA MATa his3-1 leu2-0 met15-0
