Kit de optimización de transfección de células primarias para Nucleofector 4D unidad X, 1 kit de 96 reacciones

The Primary Cell Optimization 4D-Nucleofector^TM X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector^TM X Unit.

With the unique capability of the 4D-Nucleofector^TM X Unit to address individual wells of a 16-well Nucleocuvette^TM Strip with different programs, primary cell optimizations are easily performed within one experiment.

Complete Optimization: Using five 16-well Nucleocuvette^TM Strips and our pmaxGFP^TM Vector as positive control, our five Primary Cell Nucleofector^TM Solutions P1 – P5 are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette^TM Strip can be used for optional fine tuning or performing the first experiment with the selected conditions. Cell Group-Specific Optimization using

Cell Group-Specific Optimization using Basic Protocols: In addition to a complete optimization described above, we also provide a cell group-specific optimization protocol for some primary cell groups. Those so called Basic Protocols refer to a pre-selected set of 1 or 2 Primary Cell Nucleofector^TM Solutions and Nucleofector^TMPrograms. Basic Protocols are available for following cell groups:

• Endothelial cells (mammalian)
• Epithelial cells (mammalian)
• Fibroblasts (mammalian)
• Glial cells (mammalian)
• Neurons (mammalian)
• Smooth muscle cells (mammalian)
• Stem cells (human)

Benefits

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
• Optimal Nucleofection conditions determined in the 16-well Nucleocuvette^TM Strips are transferable to the 100 μL single Nucleocuvette^TM in the 4D-Nucleofector^TM X Unit
• Conditions are transferable between 4D-Nucleofector^TM System, 96-well Shuttle^TMSystem and 384-well Nucleofector^TM System

Applications

• Determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector^TM X Unit