Kit de optimización de transfección de líneas celulares para Nucleofector 4D unidad X, 1 kit de 64 reacciones

The Cell Line Optimization 4D-Nucleofector^TM X Kit is the ideal tool to conveniently and rapidly determine Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector^TM X Unit.

With the unique capability of the 4D-Nucleofector^TM X Unit to address individual wells of a 16-well Nucleocuvette^TM Strip with different programs, cell line optimizations are easily performed within one experiment. Using three 16-well Nucleocuvette^TM Strips and our pmaxGFP^TM Vector as positive control, our three Cell Line Nucleofector^TMSolutions SE, SF and SG are tested in combination with a pre-selected set of programs plus controls. The remaining 16-well Nucleocuvette^TM Strip can be used for optional fine tuning or performing the first experiment with the selected conditions.

 Benefits

• Convenient and rapid determination of optimal Nucleofection conditions for a broad range of primary cells within one experiment
• Optimal Nucleofection conditions determined in the 16-well Nucleocuvette^TM Strips are transferable to the 100 μL single Nucleocuvette^TM in the 4D-Nucleofector^TM X Unit
• Conditions are transferable between 4D Nucleofector^TM System, 96-well Shuttle^TMSystem and 384-well Nucleofector^TM System

Applications

• Determination of Nucleofection conditions for cell lines lacking an Optimized Protocol for the 4D-Nucleofector^TM X Unit