KIT DIAGNÓSTICO ELISA, POTY GROUP PARA 480 ENSAYOS, 1 KIT

The antibodies are monoclonal (As-057371) and were raised against the peanut stripe virus isolate of Bean common mosaic virus (3, and H.J. Vetten, personal communication). They have been shown to react with a large number of viruses of the genus Potyvirus and also with some members of the genus Rymovirus (3). However, they did not react with members of the genera Bymovirus, Ipomovirus and Tritimovirus (3). Homogenize a plant sample of approx. 0.1 g in 5 ml extraction buffer (1:50 w/v) (3) in the special extraction buffer «PTA». With certain samples (e.g. tobacco), further dilution to 1:500 (w/v) in extraction buffer gives even stronger results. On the other hand, more concentrated extracts (1:10 - 1:20, w/v) generally give weaker results. The product was developed in cooperation with Biologische Bundesanstalt für Land- und Forstwirtschaft (BBA), Braunschweig, Germany