A pooled lentiviral microRNA screen can be performed to identify microRNAs that regulate development and signaling pathways, without the need for costly high-throughput automation.
Innovative vector design for robust microRNAs robust expression from shMIMIC Inducible Lentiviral microRNAs
SMARTchoice options let you select the promoter and reporter for optimal performance in your experiment
Tight regulation of expression from the Tet-On® 3G transactivator protein
Choose shMIMIC Lentiviral microRNA Pooled Libraries for constitutive expression of mature microRNAs.
Each shMIMIC lentiviral library includes:
30 Non-targeting controls
80 species-specific positive control shRNAs targeting 10 common viability and reference genes (8 shRNAs each)
A data file including construct sequences, miRBase gene IDs, and counts per millions of mapped reads
1Total constructs includes 110 non-targeting and gene-specific shRNA constructs. All shMIMIC Lentiviral microRNA pooled libraries are ≥ 5 × 108 TU/mL (+/- 20 %) provided in 8 tubes × 25 µL (200 µL total)
shMIMIC microRNA Pooled Library
Number of mature microRNAs
Number of unique designs
Number of pools × number of constructs per pool1
Human 2580 2555 1 pool × 2665 constructs
Mouse 1913 1896 1 pool × 2006 constructs
Human-Mouse Conserved 355 human + 341 mouse 386 1 pool x 496 constructs
Custom collections of microRNA pooled libraries are available
Request a custom collection of human, mouse, or rat microRNAs
Available pool sizes are between 50 to 10,000 constructs
Pools are provided as purified, concentrated lentiviral particles
≥ 5 x 108 TU/mL for constitutive vectors and ≥ 1 x 107 TU/mL for inducible vectors
Request pricing for a custom pooled library
Products recommended in our validated protocol:
Vector-matched SMARTvector Inducible Non-targeting control for transduction optimization
SMARTvector Indexing PCR and Sequencing Primer Kits (A & B) with 12 unique indexing primers, optimized and experimentally validated for: Efficient PCR amplification of genomic DNA with minimal bias
High-throughput multiplexed Next Gen Sequencing for hit identification
Caracteristicas
Tight control of microRNA expression for powerful functional screening of hundreds or thousands of microRNAs.
